Abstract P22: Requirement Of talin1 For Cell Proliferation During Palate And Mandibular Development In Zebrafish

RESULTS: The hMSCs in HCS still maintain self-renewal and differentiation capacity after extended periods of culture. The TGF-β1, ANG1 and ANG2 amount in PHCS was higher than HCS (p<0.05) whereas the VEGF amount in PHCS was significantly lower than that in HCS (p<0.05). The HCS and the PHCS implantation significantly reduced skin contraction and improved cosmetic appearance relative to the STSG control group. The PHCS group experienced the least hemorrhage and necrosis, and lowest inflammatory cell infiltration. It also induced the highest neovascularization in early stages, which established a robust blood microcirculation to support grafts survival and tissue regeneration. Moreover, the PHCS grafts preserved the largest amount of skin appendages (including hair follicles and sebaceous glands) and developed the smallest epidermal thickness. The collagen deposition and fibril morphology in PHCS grafts were also similar to normal skin, indicating a lower degree of skin fibrosis. The superior therapeutic effect seen in PHCS groups was attributed to the elevated presence of growth factors and cytokines in the pre-vascularized cell sheet, which exerted a beneficial paracrine signaling during the early stage of wound repair.